Adding Diet and Exercise Counseling to the Health Promotion Plan Alleviates Anthropometric and Metabolic Complications in Patients with Metabolic Syndrome

Abstract

We investigated the effects of individual (IC) and group (GC) diet and exercise counseling in men with metabolic syndrome. Participants received exercise instruction and exercise load was monitored. IC participants received individual diet counseling sessions and general consultations at baseline and monthly. GC participants received a group diet counseling session at baseline and general consultations at baseline and monthly. In the IC group, body mass index (BMI) percent body fat, waist circumference, diastolic blood pressure, low-density lipoprotein cholesterol, glycosylated hemoglobin A_1c, and liver function levels were reduced significantly after 3 months, whereas in the GC group, waist circumference and levels of liver function were reduced. Exercise load was negatively correlated with change in BMI and waist circumference in the IC group, and positively correlated with changes in high-density lipoprotein cholesterol levels in all subjects and in the GC group. Diet and exercise counseling, especially IC, may benefit patients with metabolic syndrome.

Keywords

counsel diet exercise metabolic syndrome waist circumference

Introduction

Obesity has recently emerged as a global health concern. Indeed, patients with metabolic syndrome are now recognized as a high-risk group for cardiovascular diseases (CVD), next to patients with hypercholesterolemia.^1,2 Metabolic syndrome involves having a clinical disorder based on abdominal obesity, including atherogenic dyslipidemia, elevated blood pressure, and high blood glucose and/or insulin resistance. In Japan, a report from the Ministry of Health, Labor and Welfare showed an increase in the number of individuals with metabolic syndrome, associated with the westernization of lifestyle.³ It has been demonstrated that individuals with a combination of 3 or more risk factors out of obesity, hyperglycemia, hyperlipidemia and hypertension had a 10.56-times increased relative risk for acute myocardial infarction.⁴ In addition to the above-mentioned classical risk factors, obesity is associated with several co-morbidities such as sleep apnea/sleep-disordered breathing and nonalcoholic fatty liver disease, which are themselves recognized as causes of CVD.^4–6 For exsample, recent epidemiological data suggest that gamma-glutamyl transferase (GGT) is not only a biomaker of hepatobiliary diseases, but also a surrogate marker of metabolic and cardiovascular risk^7–10.

Based on these evidences, It is necessary to take measures to prevent CVD in patients with metabolic syndrome.

A change in lifestyle that includes alterations in diet and exercise can not only improve glucose tolerance^11–13 but also reduce the magnitude of several other cardiovascular risk factors such as obesity, dyslipidemia, high blood pressure,^14–16 and metabolic syndrome,^17,18 thereby decreasing the risk of CVD. Treatment recommendations in patients with nonalcoholic fatty liver disease, for example, include weight reduction through both diet and physical activity, in addition to weight-loss surgery for extreme obesity.¹⁹ Nutritional therapy may also be beneficial and cost-effective, although there have been no convincing randomized controlled trials investigating the effects of nutritional education in patients with metabolic syndrome or obesity.^20,21

It is often difficult for patients to change their behaviors and environment. Henkin and colleagues²² suggested that lack of long-term compliance to the reduction of dietary saturated fat remains a problem, even in individuals who receive intensive initial training and show early favorable responses. Effective and useful methods for nutrition and exercise education included as part of a total health promotion plan are therefore necessary in order to improve metabolic disorders. A public health program that includes group-based or individual counseling could provide this. Individual counseling is reported to be effective in the prevention of type 2 diabetes mellitus,^11,14 and group-based diabetes education programs have been shown to result in clinical improvements.¹⁵

In this study, we assessed two types of diet and exercise counseling—individual counseling (IC) and group counseling (GC)—and evaluated the effects of each type of counseling on various metabolic parameters, including anthropometric profiles [body mass index (BMI),percent body fat, waist circumference, and blood pressure], lipid and glucose metabolism, and hepatic function. We hypothesized that IC is more effective than GC. The goal of this study was to determine the extent to which each type of counseling can improve physical profiles and metabolic disorders in patients with metabolic syndrome, in order to plan an effective total health promotion plan for company employees.

Method

Participants

The study group consisted of patients with metabolic syndrome who underwent an employee health checkup at their workplace between April 2005 and March 2006. A total of 2,295 employees were screened. In accordance with the definition by the Japanese Committee for the Diagnostic Criteria of Metabolic Syndrome,²³ metabolic syndrome was defined as the presence of visceral obesity (waist circumference = 85 cm or more in men) and 2 or more of the following abnormalities: (i) triglycerides ≥ 150 mg/dL and/or high-density lipoprotein cholesterol (HDL-C) < 40 mg/dL, (ii) systolic blood pressure (SBP) ≥ 130 mmHg and/or diastolic blood pressure (DBP) ≥ 85 mmHg, and (iii) fasting glucose ≥ 110 mg/dL. Individuals were excluded from the study if they suffered from malignant diseases, inflammatory diseases, a history of atherosclerotic or cardiac diseases, virus or autoimmune hepatitis and cirrhosis, renal diseases, thyroid diseases, arthropathy or other physical problems. Patients with medication-treated diabetes mellitus, dyslipidemia, and primary or secondary hypertension were also excluded.

The study protocol was explained to 161 men who were diagnosed with metabolic syndrome and randomized into two groups. Prior to the start of the study, some participants dropped out for personal reasons that included loss of interest in the study, work transfer, change in schedule and other unknown causes. Ultimately, 37 and 54 men were enrolled in the IC and GC groups respectively. Informed consent was obtained from all study participants. The study protocol was approved by the Human Ethics Committee of Shinko Kakogawa Hospital (presently Kakogawa East City Hospital) and carried out according to the Declaration of Helsinki.

Apparatus

Blood pressure was measured while each participant was in a seated position using an automatic electronic sphygmomanometer (BP-203RV II, Nihon COLIN Corporation, Aichi, Japan). BMI was calculated by dividing body weight by the square of height (kg/m²). Percent body fat was determined by bioelectrical impedance analysis using TANITA BC-118E (TANITA Corporation, Tokyo, Japan). Waist circumference at the umbilical level was measured in the late exhalation phase in a standing position.^24,25 Alcohol consumption was calculated as grams per week from each participant's interview, and estimated as average alcohol intake (in grams) per day.

Study protocol

The goal of this study was to evaluate the effect of diet and exercise counseling on various metabolic parameters, and to investigate the extent to which individual or group counseling can improve these metabolic parameters in patients with metabolic syndrome. Each participant was assessed for 3 months.

To encourage physical activity, at baseline and once monthly (4 times in total), all study participants received exercise instruction that included advice to maintain caloric expenditure above 300 kcal a day. To estimate exercise load by daily walking, study participants were monitored using a step-counter (KENZ lifecorder EX, SUZUKEN Co., Nagoya, Japan), which automatically calculated daily energy consumption (in kcal units). Data were collected at every session. Overtime work from 0 to 40 hours per month was recorded, yielding no difference between the IC group and the GC group. Since exercise load differed by approximately 100 to 350 kcal depending on the type of occupation, we did not separately evaluate exercise during working and non-working time, but rather measured the load in a whole day.

For diet therapy in the IC group, participants were asked to record their diet, alcohol consumption, and timing of meals for three consecutive days before the session at baseline and once monthly. These diet records were analyzed by dietitians. Based on dietitian reports, participants received individually tailored 1-hour counseling sessions, and were advised to maintain a well-balanced, low-fat diet with adequate calories. In addition, participants in the IC group received individual 1-hour general consultations regarding exercise and lifestyle by physicians and public health nurses at baseline and once monthly (4 times in total).

Participants in the GC group did not receive individual dietary analysis but participated in a 1-hour group session that included fundamental dietary advice by dietitians at baseline only. Additionally, individual general consultations by physicians and public health nurses were provided at baseline and once monthly (4 times total).

Body weight, percent body fat, and waist circumference were each measured by registered nurses at baseline and monthly. Blood sampling and analysis were performed at the beginning of the study and three months later.

Blood sampling and analysis

Blood samples were obtained between 9 AM and 11 AM following a 12-hour fast. Plasma triglycerides, HDL-C, total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C, Kyowa Medex, Tokyo, Japan), aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and GGT levels were determined by enzymatic methods, fasting plasma glucose and glycosylated hemoglobin A_1c (HbA_1c) by high-performance lipid chromatography (HPLC), high-sensitivity C-reactive protein (hsCRP) by a nephelometry method, and fasting plasma insulin levels by enzyme immunoassay. In accordance with homeostasis model assessment (HOMA),²⁶ insulin resistance (HOMA-IR) was calculated as fasting plasma glucose (mg/dL) × fasting insulin (μU/mL)/405.

Statistical analysis

Continuous variables were expressed as the mean ± standard deviation (SD) and analyzed using Student's t-test or Pearson's chi-square test as appropriate. Correlations were calculated using the formula for Pearson's correlation coefficient. Probability values of less than 0.05 were considered statistically significant. All data were analyzed using Statview software version 4.0 (Abacus, Berkeley, CA, USA).

Results

Comparison of metabolic profiles between ic group and gc groups

There were no dropouts in either group throughout the course of the present study. Baseline characteristics of all participants are summarized in Table 1. Although there were no differences in anthropometric profiles, blood pressure, and lipid parameters between the IC and GC groups at baseline, the GC group showed significantly higher levels of AST, ALT, and cholinesterase. By contrast, the GC group showed significantly lower levels of HOMA-IR, HbA_1c, and hsCRP. Since there were differences in some metabolic parameters at baseline between IC and GC groups, we compared the data before and after the study within each group.

Table 1.

Baseline characteristics of patients in IC and GC groups.

	IC (n = 37)	GC (n = 54)
Age (years)	51.1 ± 6.4	49.6 ± 5.0
Alcohol intake (g/day)	20.1 ± 18.8	21.7 ± 18.2
BMI (kg/m²)	27.3 ± 2.1	27.6 ± 2.3
Percent body fat (%)	31.0 ± 4.5	30.2 ± 4.4
Waist circumference (cm)	94.1 ± 5.2	95.3 ± 5.7
SBP (mmHg)	135.5 ± 15.9	136.1 ± 16.6
DBP (mmHg)	85.9 ± 9.9	87.8 ± 12.3
TC (mg/dL)	213.6 ± 30.1	210.8 ± 28.6
LDL-C (mg/dL)	130.4 ± 27.5	128.0 ± 26.7
HDL-C (mg/dL)	49.2 ± 10.3	49.9 ± 10.9
Triglycerides (mg/dL)	183.4 ± 84.5	191.6 ± 115.2
Plasma glucose (mg/dL)	103.8 ± 15.6	99.3 ± 11.6
Plasma insulin (μU/ml)	7.5 ± 5.5	5.9 ± 2.0
HOMA-IR	1.93 ± 1.60	1.45 ± 0.48^a
HbA_1c (%)	5.43 ± 0.58	5.20 ± 0.48^a
AST (IU/l)	26.4 ± 8.3	34.7 ± 12.7^b
ALT (IU/l)	32.8 ± 18.2	47.4 ± 21.7^b
ALP (U/l)	251.5 ± 64.4	254.7 ± 85.8
GGT (IU/l)	60.0 ± 38.6	86.7 ± 79.5
Cholinesterase (IU/l)	364.1 ± 61.7	404.5 ± 73.9^c
hsCRP (mg/l)	0.25 ± 0.67	0.11 ± 0.17^d

Notes: Values are the mean ± SD.

P < 0.0002;

P < 0.0005;

P < 0.01;

P < 0.05 vs. IC participants (unpaired Student's t-test).

Abbreviations: IC, individual counseling; GC, group counseling; BMI, body mass index; SBP, systolic blood pressure; DBP, diastolic blood pressure; TC, total cholesterol; LDL-C, low-density lipoprotein cholesterol; HDL-C, high-density lipoprotein cholesterol; HOMA-IR, fasting plasma glucose (mg/dL) × fasting insulin (μU/mL)/405; HbA_1c, glycosylated hemoglobin A_1c; AST, aspartate aminotransferase; ALT, alanine aminotransferase; ALP, alkaline phosphatase; GGT, gamma-glutamyl transpeptidase; hsCRP, high-sensitivity C-reactive protein.

Table 2 shows the effect of individual and group counseling on alcohol intake, anthropometric profiles, and blood pressure after 3 months. Alcohol intake did not change in either group. We could not assess exercise load before the study. After 3 months exercise load in the IC group appeared to be slightly greater than that in the GC group, but was not significantly different. In the IC group, BMI, percent body fat, waist circumference, and DBP levels were significantly reduced over time. A significant reduction in waist circumference was also observed in the GC group.

Table 2.

Age, exercise load, alcohol intake, body weight, body mass index, waist circumference, and blood pressure in IC and GC groups.

	IC (n = 37)		GC (n = 54)
	Pre	Post	Pre	Post
Age (years)	51.1 ± 6.4	–	49.6 ± 5.0	–
Alcohol intake (g/day)	20.1 ± 18.8	17.9 ± 17.6	21.7 ± 18.2	21.7 ± 18.5
Exercise load (kcal/day)	–	373.4 ± 111.3	–	329.0 ± 113.3
BMI (kg/m²)	27.3 ± 2.1	26.7 ± 2.0^a	27.6 ± 2.3	27.5 ± 2.5
Percent body fat (%)	31.0 ± 4.5	28.5 ± 4.3^a	30.2 ± 4.4	30.6 ± 4.5^d
Waist circumference (cm)	94.1 ± 5.2	91.9 ± 4.9^a	95.3 ± 5.7	93.8 ± 5.6^c
SBP (mmHg)	135.5 ± 15.9	132.2 ± 16.8	136.1 ± 16.6	139.5 ± 14.9^d
DBP (mmHg)	85.9 ± 9.9	82.3 ± 9.8^b	87.8 ± 12.3	88.4 ± 14.3^d

Notes: Values are the mean ± SD.

vs. pre-IC participants at P < 0.0002;

vs. pre-IC participants at P < 0.02;

vs. pre-GC participants at P < 0.0001 (paired Student's t-test);

vs. post-IC participants at P < 0.03 (unpaired Student's t-test).

Abbreviations: IC, individual counseling; GC, group counseling; Pre, at baseline; Post, after study; BMI, body mass index; SBP, systolic blood pressure; DBP, diastolic blood pressure.

As shown in Table 3, plasma levels of LDL-C and HbA_1c were significantly reduced in the IC group. In contrast, plasma levels of insulin and HOMA-IR increased significantly in the GC group. In both groups, AST, ALT, GGT, and cholinesterase levels were significantly reduced.

Table 3.

Plasma analyses in IC and GC groups.

	IC (n = 37)		GC (n = 54)
	Pre	Post	Pre	Post
TC (mg/dL)	213.6 ± 30.1	208.6 ± 32.0	210.8 ± 28.6	214.0 ± 36.8
LDL-C (mg/dL)	130.4 ± 27.5	122.5 ± 29.2^*	128.0 ± 26.7	132.2 ± 31.4
HDL-C (mg/dL)	49.2 ± 10.3	49.7 ± 11.2	49.9 ± 10.9	49.5 ± 11.0
Triglycerides (mg/dL)	183.4 ± 84.5	193.7 ± 141.2	191.6 ± 115.2	176.7 ± 112.7
Plasma glucose (mg/dL)	103.8 ± 15.6	104.5 ± 21.4	99.3 ± 11.6	105.1 ± 16.0
Plasma insulin (μU/mL)	7.5 ± 5.5	7.5 ± 6.3	5.9 ± 2.0	7.9 ± 4.9^†,b
HOMA-IR	1.93 ± 1.60	1.88 ± 1.52	1.45 ± 0.48^a	2.02 ± 1.16
HbA_1c (%)	5.43 ± 0.58	5.36 ± 0.55^*	5.20 ± 0.48^a	5.27 ± 0.55
AST (IU/l)	26.4 ± 8.3	23.3 ± 5.9^*	34.7 ± 12.7^a	32.0 ± 11.2^†,b
ALT (IU/l)	32.8 ± 18.2	25.6 ± 11.1^*	47.4 ± 21.7^a	42.9 ± 21.8^†,b
ALP (U/l)	251.5 ± 64.4	251.9 ± 75.9	254.7 ± 85.8	247.2 ± 71.8
GGT (IU/l)	60.0 ± 38.6	50.2 ± 32.3^*	86.7 ± 79.5	77.5 ± 62.5^†,b
Cholinesterase (IU/l)	364.1 ± 61.7	343.6 ± 63.9^*	404.5 ± 73.9^a	387.2 ± 62.3^†,b
hsCRP (mg/l)	0.25 ± 0.67	0.28 ± 0.74	0.11 ± 0.17^a	0.12 ± 0.15

Notes: Values are the mean ± SD.

vs. pre-IC participants at P < 0.05;

†

vs. pre-GC participants at P < 0.05 (paired Student's t-test).

vs. pre-IC participants at P < 0.05;

vs. post-IC participants at P < 0.05 (unpaired Student's t-test).

Abbreviations: IC, individual counseling; GC, group counseling; Pre, at baseline; Post, after study; TC, total cholesterol; LDL-C, low-density lipoprotein cholesterol; HDL-C, high-sensitivityβ lipoprotein cholesterol; HOMA-IR, fasting plasma glucose (mg/dL) × fasting insulin (μU/mL)/405; HbA_1c, glycosylated hemoglobin A_1c; AST, aspartate aminotransferase; ALT, alanine aminotransferase; ALP, alkaline phosphatase; GGT, gamma-glutamyl transpeptidase; hsCRP, high-sensitivity C-reactive protein.

To confirm whether there was an increased effectiveness of IC versus GC, we computed a change score (Δ) for each parameter in Tables 2 and 3 by subtracting the value at baseline from the value after 3 months. Among these parameters, ΔBMI, Δ percent body fat, ΔSBP, ΔLDL-C, ΔHOMA-IR, and ΔHbA_1c were each significantly greater in IC than in GC (Fig. 1). While both types of counseling were somewhat effective, these results suggest that IC led to the greatest improvement in various metabolic parameters.

Figure 1.

Comparison of differences in various parameters between IC and GC using an unpaired Student's t-test.

To further evaluate the efficacy of IC and GC, we determined the number of participants who had initially been diagnosed with metabolic syndrome at baseline and still met the defined criteria for the disease at the end of the study. Table 4 shows the decrease in the number of participants who met the criteria of metabolic syndrome, greater waist circumference, dyslipidemia, high blood pressure, or high plasma glucose. Results suggest that both IC and GC improved various clinical factors in metabolic syndrome. Among these factors, the decrease in the number of participants with high blood pressure in the IC group was significantly different than that in the GC group.

Table 4.

Number of participants with metabolic syndrome, greater waist circumference, dyslipidemia, high blood pressure, or high plasma glucose in IC and GC groups.

	IC		GC		P
	Pre	Post	Pre	Post	P
Metabolic syndrome	37	20	54	31	NS
Greater waist circumference	37	36	54	52	NS
Dyslipidemia	24	19	33	23	NS
High blood pressure	27	18	41	36	0.035
High plasma glucose	17	11	12	9	NS

Notes: The data represent numbers of participants with metabolic syndrome, greater waist circumference, dyslipidemia, high blood pressure, or high plasma glucose. Metabolic syndrome was defined as the presence of 2 or more abnormalities in addition to visceral obesity (greater waist circumference: 85 cm or more in men). These three abnormalities are as follows: (i) dyslipidemia; triglycerides ≥ 150 mg/dL and/or high-density lipoprotein cholesterol (HDL-C) < 40 mg/dL, (ii) high blood pressure; systolic blood pressure (SBP) ≥ 130 mmHg and/or diastolic blood pressure (DBP) ≥ 85 mmHg, (iii) high plasma glucose; fasting glucose ≥ 110 mg/dL. Pearson's chi-square test.

Moreover, we examined the number of participants who showed improvements in metabolic parameters (as indicated by a decreased value, except for HDLC) in both groups. The proportion of participants exhibiting a lower BMI was higher in the IC group than in the GC group (IC: n = 32/37; GC: n = 28/54; χ² = 11.73, P = 0.0006; data not shown). Similarly, IC resulted in an increased proportion of individuals with loss of percent body fat (IC: n = 32/37; GC: n = 23/54; χ² = 17.69; P < 0.0001), decreased LDL-C (IC: n = 28/37; GC: n = 23/54; χ² = 9.76, P = 0.002), decreased plasma glucose (IC: n = 20/37; GC: n = 13/54; χ² = 8.54, P = 0.004), decreased HbA_1c (IC: n = 19/37; GC: n = 12/54; χ² = 8.29, P = 0.004), decreased plasma insulin (IC: n = 19/37; GC: n = 14/54; χ² = 6.14, P = 0.013), and decreased HOMA-IR (IC: n = 21/37; GC: n = 14/54; χ² = 8.82, P = 0.003).

Relationship between exercise load and various parameters

Correlation analyses demonstrated that exercise load was negatively correlated with ΔBMI and Δ percent body fat, and positively correlated with ΔHDL-C in all participants (total; Table 5). In the IC group, exercise load was negatively correlated with ΔBMI and Δ waist circumference. Exercise load was positively correlated with ΔHDL-C in the GC group. In addition, alcohol intake was weakly but significantly correlated with GGT (r = 0.302, P = 0.004) and LDL-C (r = 0.214, P = 0.044).

Table 5.

The correlation between exercise load and differences in various parameters in total, IC, and GC groups.

	Total		IC		GC
	r	P	r	P	r	P
ΔBMI (kg/m²)	-0.323	0.002	-0.480	0.003	-0.104	NS
ΔPercent body fat (%)	-0.227	0.030	-0.185	NS	-0.118	NS
ΔWaist circumference (cm)	-0.179	NS	-0.328	0.048	-0.039	NS
ΔSBP (mmHg)	-0.019	NS	0.080	NS	-0.006	NS
ΔDBP (mmHg)	0.142	NS	0.255	NS	0.152	NS
ΔTC (mg/dL)	-0.052	NS	-0.020	NS	-0.020	NS
ΔLDL-C (mg/dL)	-0.066	NS	-0.183	NS	0.076	NS
ΔHDL-C (mg/dL)	0.292	0.005	0.271	NS	0.304	0.026
ΔTriglycerides (mg/dL)	-0.152	NS	-0.085	NS	-0.266	NS
ΔPlasma glucose (mg/dL)	-0.039	NS	0.013	NS	-0.006	NS
ΔPlasma insulin (μUI/mL)	-0.161	NS	-0.142	NS	-0.124	NS
ΔHOMA-IR	-0.155	NS	-0.114	NS	-0.123	NS
ΔHbA_1c (%)	-0.107	NS	-0.016	NS	-0.076	NS
ΔAST (IU/l)	-0.062	NS	-0.136	NS	-0.030	NS
ΔALT (IU/l)	-0.136	NS	-0.191	NS	-0.085	NS
ΔALP (U/l)	-0.059	NS	-0.065	NS	-0.096	NS
ΔGGT (IU/l)	0.110	NS	-0.150	NS	0.230	NS
ΔCholinesterase (IU/l)	-0.101	NS	-0.074	NS	-0.104	NS
ΔhsCRP (mg/l)	-0.088	NS	-0.216	NS	0.069	NS

Note: Δ in each parameter refers to the difference determined by subtracting the baseline value from the value after diet and exercise.

Abbreviations: IC, individual counseling; GC, group counseling; Pre, at baseline; Post, after study; BMI, body mass index; SBP, systolic blood pressure; DBP, diastolic blood pressure; TC, total cholesterol; LDL-C, low-density lipoprotein cholesterol; HDL-C, high-sensitivity lipoprotein cholesterol; HOMA-IR, fasting plasma glucose (mg/dL) × fasting insulin (μU/mL)/405; HbA_1c, glycosylated hemoglobin A_1c; AST, aspartate aminotransferase; ALT, alanine aminotransferase; ALP, alkaline phosphatase; GGT, gamma-glutamyl transpeptidase; hsCRP, high-sensitivity C-reactive protein.

Discussion

In this study, we demonstrated that with the exception of TG and HDL-C levels, IC and GC improved various clinical factors in metabolic syndrome. These results suggest that counseling for lifestyle change is an effective treatment for metabolic syndrome. Results implicated IC in particular in being a more effective treatment than GC. The lack of significant change found in TG and HDL-C levels may be due to continual intake of alcohol throughout the study, or to a non-significant difference in exercise loads between the two groups.

Compliance to behavior modifications in diet and physical activity is an important component of all treatments, and the role of therapeutic intervention in this process is not clearly established.^27,28 It is known that some patients do not comply with dietary advice, while others might only maintain strict dietary control for a short time. Thus, adherence to dietary treatments is difficult to maintain and diet trials have been reported to have a high rate of dropout.^29,30

It has been reported that the dropout rate of overweight patients consulted solely by dietitians (45%) was significantly greater than that of patients consulted by a doctor and dietitian (29%).²⁹ Thompson and colleagues³⁰ reported that patients with hypercholesterolemia receiving advice from dietitians experienced a greater reduction in total blood cholesterol than those receiving advice from doctors, suggesting that dietitians, as well-trained nutritional staff, can motivate and provide higher-quality diet intervention for patients requiring treatment. In the present study, the greatest advantage for study participants in the IC compared to the GC group was the opportunity for individual analysis of consecutive 3-day food records by a dietitian, as described in the study protocol. Participants in the IC group were given nutritional analysis sessions by dietitians followed by consultation with physicians and public health nurses, resulting in a greater improvement in metabolic disorders compared with those in the GC group. IC may enable health practitioners to give specific and appropriate advice, foster valuable discussion, and focus on concrete methods to improve metabolic parameters in each participant, resulting in a high motivation to maintain changes in diet and physical activity.

Some prospective studies have demonstrated that cardiorespiratory fitness is associated with the incidence of metabolic syndrome.^31,32 As shown in Table 5, exercise load in the present study was negatively correlated with ΔBMI and Δ percent body fat, and positively correlated with ΔHDL-C in all participants. This may suggest that purposeful exercise in addition to regular work is effective in treating metabolic disorders in company employees. Walking is the most common form of physical activity. It is safe and easy, and improves a number of parameters of health. Therefore, brisk walking for at least 30 minutes daily can be recommended as the principal form of physical activity at the population level.^33,34 The level of exercise load in our study was adequate to see improvement (373.4 kcal/day in the IC group and 329.0 kcal/day in the GC group, equivalent to around 10,000 steps a day), although an exercise load in excess of those observed in the present study may result in a greater amelioration of a number of metabolic factors. Proper and colleagues³⁵ reported that individual face-to-face and repeated counseling sessions in the workplace positively influenced physical activity levels. In the current study, no differences in exercise levels at baseline were observed between the IC and GC groups, and there was no difference in exercise load per day between the two groups at the end of the study.

Although IC was more effective than GC for improvement of a number of parameters in the present study, IC is more costly and requires more dietitians than GC. The reason for the elevation in plasma insulin levels and HOMA-IR observed in the GC but not in IC group are unknown. However, as mentioned above, GC is an effective method for decreasing waist circumference. A combination therapy of GC and IC may produce longer-term benefits and be more cost-effective.

Henkin and colleagues²² demonstrated that serum lipid levels tend to return to basal levels after 6 months, even in individuals who receive intensive initial training and show an early favorable response. A systematic review of randomized controlled trials showed that although low-carbohydrate/high-protein diets have effects on reducing weight in obese persons, regain of weight is typically initiated after 6-12 months.³⁶ Frequent assessment and repetition are needed to ensure patient understanding and to maintain patient motivation and the effects of health promotion. Thus, an example plan would include analyses of consecutive 3-day dietary records, anthropometric measures, blood analyses, and instruction and advice about exercise at baseline and at 3 months (twice total), combined with individual dietary consultation, and instruction and advice about exercise at 1 and 2 months.

It has been reported that moderate intensity aerobic exercise helps to normalize ALT levels in patients with nonalcoholic steatohepatitis.³⁷ Reports from epidemiological studies also showed that an increase in serum GGT predicted onset of metabolic syndrome, incidence of CVD, and death, suggesting that GGT is a marker of metabolic and cardiovascular risk.^7–10 Our results suggest that an exercise load of around 350 kcal/day may improve AST, ALT, GGT, and cholinesterase levels, if it is accompanied by dietary counseling.

Inflammation is a key mechanism in the development of cardiovascular and liver diseases and metabolic syndrome.^1,6 In the present study, however, there was no significant improvement in hsCRP in either group. It has been reported that change in hsCRP concentration is related to changes in waist circumference and lipid metabolism, reflected by plasma triglycerides and free fatty acid levels.³⁸ We observed no decrease in triglyceride levels in the present study, suggesting that an improvement in dyslipidemia may be necessary for the reduction of hsCRP or other inflammatory markers in metabolic syndrome.

There are clear limitations in this study. As described in the Participants section, we explained the study protocol to all participants, inquired as to whether they were interested and obtained their acceptance to participate in the study. A number of individuals, however, cancelled prior to receiving counseling because the study protocol was not what they wanted, or because they were no longer able to participate due to a work transfer, change in working hours (daytime, evening, or nighttime), change in schedule, or other unknown causes. Since all participants worked for the same company, we could not reduce bias arising from a lack of blinding. Ultimately 37 men were enrolled in the IC group and 54 men in the GC group. These problems with randomization and unbalanced numbers of participants may have led to differences in baseline levels of metabolic factors between the two groups. To account for this, we did not compare the levels of these factors between the two groups, but rather compared the baseline and post-study data within each group. Although there were no dropouts during the study, we cannot exclude the possibility of bias. Improved planning of the protocol itself, to consider employee working styles, may improve the results.

A second major limitation in this study was that we did not perform a detailed nutrient analysis of the food records provided by the participants. Recently, Otsuka and colleagues³⁹ demonstrated that a lower intake of vitamin B6 and dietary fiber in men, or a lower intake of calcium, milk and dairy products and higher intake of cereal in women were related to the incidence of metabolic syndrome in Japanese people. A recent review indicates that the quantity and type of carbohydrate affects metabolic outcomes.⁴⁰ Thus, in addition to analysis of total energy and nutrient balance, it may be important to analyze specific dietary factors in each individual. We were also unable to monitor exercise load before the study, and did not moderate alcohol consumption. The number of participants in this study was small and study duration was short. Further study of longer duration with more participants is needed in order to develop and provide a strong protocol, including a combination of individual and group-based counseling sessions with cooperation of medical staff such as physicians, nurses, and dietitians.

In conclusion, lifestyle diet and exercise counseling, especially individually, may improve physical profiles and metabolic disorders in patients with metabolic syndrome.

Competing Interests

Authors disclose no potential conflicts of interest.

Funding

Author(s) disclose no funding sources.

Author Contributions

Experiment conception and design: S.M-S. Data analysis: S.M-S., Y.F. First draft of manuscript: S.M-S., H.M. Contribution to writing of the manuscript: Y.F., H.M., M.T., M.H. All members agreed with results and conclusions, jointly developed the structure and arguments for the paper, made critical revisions and approved the final version of the manuscript.

Disclosures and Ethics

The study protocol was approved by the Human Ethics Committee of Shinko Kakogawa Hospital and carried out according to the Declaration of Helsinki. As a requirement of publication author(s) have provided to the publisher signed confirmation of compliance with legal and ethical obligations including but not limited to the following: authorship and contributorship, conflicts of interest, privacy and confidentiality and protection of human research subjects. The authors have read and confirmed their agreement with the ICMJE authorship and conflict of interest criteria. The authors have also confirmed that this article is unique and not under consideration or published in any other publication, and that they have permission from rights holders to reproduce any copyrighted material. Any disclosures are made in this section. The external blind peer reviewers report no conflicts of interest.

Footnotes

Acknowledgements

We wish to thank physicians Kenzo Yamashiro, Yoshio Ohnishi, and Isao Utaka, as well as Noriko Taitani, a public health nurse, and Rumiko Taniguchi, a registered nurse, dietitians Kiyomi Niboshi, Takao Kameda, and Hideo Ohira, and all staff engaged in laboratory examinations in Kakogawa East City Hospital for their valuable help with this study.

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