Effect of Inulin and Lactulose on Survival of Lactobacillus AcidophilusLA-5 and Bifidobacterium Bifidum BB-02 in Acidophilus-Bifidus Yoghurt

Abstract

The effect of supplementation of lactulose and inulin as prebiotics on the growth of L. acidophilusLA-5 and B. bifidumBB-02 in Acidophilus-Bifidus (AB) yoghurt and some quality parameters of the resulting products were studied. Yoghurts produced from milks with added inulin at levels of 0.5% and 1.0%, or with lactulose at levels of 0.25% and 2.5% were compared with classical yoghurt and AB yoghurt (without added prebiotics). The results showed that inulin and lactulose did not affect the growth of yoghurt starter bacteria, but stimulated the growth of B. bifidumBB-02 to a great extent. Lactulose was found to be more effective on the growth of both probiotic strains than inulin. Inulin did not stimulate the growth of L. acidophilusLA-5. The cell counts of B. bifidumBB-02 and L. acidophilusLA-5 were dependent upon concentrations of lactulose and inulin used. No significant (p< 0.05) difference between the samples were observed with regard to the pH and lactic acid values. However, lactulose or inulin added samples had lower acetaldehyde and tyrosine contents than the untreated samples.

Keywords

yoghurt probiotics prebiotics Lactobacillus acidophilus Bifidobacterium bifidum

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References

Adhikari K. , Mustapha A. , Grün I.U. and Fernando L. (2000). Viability of microencapsulated bifidobacteria in set yogurt during refrigerated storage . Journal of Dairy Science 83: 1946–1951 .

Dave R.I. and Shah N.P. (1997). Viability of yogurt and bacteria in yogurts made from commercial starter cultures . International Dairy Journal 7: 31–41 .

Gopal P.K. , Sullivan P.A. and Smart J.B. (2001). Utilisation of galacto-oligosaccharides as selective substrates for growth by lactic acid bacteria including Bifidobacterium lactisDR10 and Lactobacillus rhamnosusDR20 . International Dairy Journal 11: 19–25 .

Guarner F. and Schaafsma G.J. (1998). Probiotics . International Journal of Food Microbiology 39: 237–238 .

Gurgun V. and Halkman K. (1990). Microbiological Counting Methods. Ankara: Society of Food Technology Publishing .

Hattingh A.L. and Viljoen B.C. (2001). Yoghurt as a probiotic carrier food . International Dairy Journal 11: 1–7

Hoier E. (1992). Use of probiotic starter cultures in dairy products . Food Australia 44: 418–420 .

Holcomb J.E. and Frank J.F. (1991). Viability of Lactobacillus acidophilusand Bifidobacterium bifidumin soft serve frozen yoghurt . Cultured Dairy Production Journal 26: 4–5 .

Holzapfel W.H. and Schillinger U. (2002). Introduction to pre and probiotics . Food Research International 35: 109–116 .

10.

Lankaputhra W.E.V. and Shah N.P. (1996). A simple method for selective enumeration of Lactobacillus acidophilusin yoghurt supplemented with L. acidophilus and Bifidobacteriumspp . Milchwissenschaft 51: 446–450 .

11.

Lees G.L. and Jago G.R. (1969). Methods for the estimation of acetaldehyde in cultured dairy products . The Australian Journal of Dairy Technology 24: 181–185 .

12.

Mann J.C. de. , Rogosa M. and Sharpe M.E. (1960). A medium for the cultivation of lactobacilli . Journal of Applied Bacteriology 23: 130–135 .

13.

Özer B.H. and Atamer M. (1999). Some properties of yoghurts produced from milk preserved by hydrogen peroxide . Milchwissenschaft 54: 628–631 .

14.

Playne M.J. and Crittenden R. (1996). Commercially available oligosaccharides . British International Dairy Federation 313: 10–22 .

15.

Rastall R. and Maitin V. (2002). Prebiotics and synbiotics: towards the next generation . Current Opinion in Biotechnology 13: 490–496 .

16.

Rybka S. (1994). The enumeration of Lactobacillus, Streptococcus-and Bifidobacteriumspecies in yoghurt. BSc Dissertation, University of New South Wales, Sydney.

17.

Rybka S. and Kalisapathy K. (1995). The survival of culture bacteria in fresh and freeze-dried AB yoghurts . Australian Journal of Dairy Technology 50: 51–57 .

18.

Rycroft C.E. , Jones M.R. , Gibson G.R. and Rastall R.A. (2001). A comparative in vitroevaluation of the fermentation properties of prebiotic oligosaccharides . Journal of Applied Microbiology 91: 878–887 .

19.

Saarela M. , Hallamaa K. , Sandholm T.M. and Matt J. (2003). The effect of lactose derivatives lactulose, lactitol and lactobionic acid on the functional and technological properties of potentially probiotic Lactobacillus strains . International Dairy Journal 13: 291–302 .

20.

Shah N.P. and Lankaputhra W.E.V. (1997). Improving viability of Lactobacillus acidophilusand Bifidobacteriumspp. in yoghurt . International Dairy Journal 7: 349–356 .

21.

Shankar P. and Davies F. (1976). Associative bacterial growth in yoghurt starters: Initial observations on stimulatory factors . Journal of Society of Dairy Technology 30: 31–32 .

22.

Singh J. , Khanna A. and Chandar H. (1980). Effect of incubation temperature and heat treatments of milk from milk of cow and buffalo on acid and flavour production by S. thermophilusand L. bulgaricus . Journal of Food Protection 43: 399–400 .

23.

Steel R.G.D. and Torrie J.H. (1980). Principles and Procedures of Statistics. New York: McGraw-Hill Book Co., Inc.

24.

Tamime A.Y. and Robinson R.K. (1999). Yoghurt Science and Technology. Cambridge: Woodhead Publishing .

25.

Tamime A.Y. , Marshall V.E. and Robinson R.K. (1995). Microbiological aspects of milks fermented by Bifidobacteria .Journal of Dairy Research 62: 151–187 .

26.

Terzaghi i B.E. and Sandine W.E. (1975). Improved medium for lactic streptococci and their bacteriophages . Applied Microbiology 29: 807–813 .

27.

Tunail N. (1978). Studies on some physical properties of selected lactic acid bacteria isolated from white brined cheeses and of lactic acid bacteria used as a starter culture. Associate Professorship Thesis. Ankara: Ankara University Press.

28.

Vinderola C.G. , Bailo J.A. and Reinheimer J.A. (2000). Survival of probiotic microflora in Argentinean yoghurt during refrigerated storage . Food Research International 33: 97–102 .