The effects of thermosonication on the quality of a stingless bee honey, the Kelulut, were studied using processing temperature from 45 to 90 ℃ and processing time from 30 to 120 minutes. Physicochemical properties including water activity, moisture content, color intensity, viscosity, hydroxymethylfurfural content, total phenolic content, and radical scavenging activity were determined. Thermosonication reduced the water activity and moisture content by 7.9% and 16.6%, respectively, compared to 3.5% and 6.9% for conventional heating. For thermosonicated honey, color intensity increased by 68.2%, viscosity increased by 275.0%, total phenolic content increased by 58.1%, and radical scavenging activity increased by 63.0% when compared to its raw form. The increase of hydroxymethylfurfural to 62.46 mg/kg was still within the limits of international standards. Optimized thermosonication conditions using response surface methodology were predicted at 90 ℃ for 111 minutes. Thermosonication was revealed as an effective alternative technique for honey processing.
AbidMJabbarSHuBHashimMMWuTLeiSet al. (2014) Thermosonication as a potential quality enhancement technique of apple juice. Ultrasonics Sonochemistry21(3): 984–990.
2.
AbidMJabbarSWuTHashimMMHuBLeiSet al. (2013) Effect of ultrasound on different quality parameters of apple juice. Ultrasonics Sonochemistry20(5): 1182–1187.
3.
AbramovičHJamnikMBurkanLKačM (2008) Water activity and water content in Slovenian honeys. Food Control19(11): 1086–1090.
4.
Abu-JdayilBAl-Majeed GhzawiAAl-MalahKIMZaitounS (2002) Heat effect on rheology of light- and dark-colored honey. Journal of Food Engineering51(1): 33–38.
5.
Ahmed J, Ramaswamy HS, Kasapis S and Boye JI. (2009). Novel Food Processing: Effects on Rheological and Functional Properties. Boca Raton, Florida: CRC Press.
6.
AlimentariusC (2001) Codex Standard for Honey, Rome: FAO, pp. 19–26.
7.
AljadiAMKamaruddinMY (2004) Evaluation of the phenolic contents and antioxidant capacities of two Malaysian floral honeys. Food Chemistry85(4): 513–518.
8.
Alvarez-SuarezJTulipaniSRomandiniSVidalABattinoM (2009) Methodological aspects about determination of phenolic compounds and in vitro evaluation of antioxidant capacity in the honey: A review. Current Analytical Chemistry5(4): 293–302.
9.
BerettaGGranataPFerreroMOrioliMMaffei FacinoR (2005) Standardization of antioxidant properties of honey by a combination of spectrophotometric/fluorimetric assays and chemometrics. Analytica Chimica Acta533(2): 185–191.
10.
Bermúdez-AguirreDBarbosa-CánovasGV (2012) Inactivation of Saccharomyces cerevisiae in pineapple, grape and cranberry juices under pulsed and continuous thermo-sonication treatments. Journal of Food Engineering108(3): 383–392.
11.
BilucaFCDella BettaFde OliveiraGPPereiraLMGonzagaLVet al. (2014) 5-HMF and carbohydrates content in stingless bee honey by CE before and after thermal treatment. Food Chemistry159: 244–249.
12.
Bogdanov S, Martin P and Lullmann C. (2002). Harmonised Methods of the International Honey Commission. Liebefeld, FAM: Swiss Bee Research Centre.
13.
Bogdanov S, Ruoff K and Persano Oddo L. (2004). Physico-chemical methods for the characterisation of unifloral honeys: A review. Apidologie 35(Suppl 1): 4–17.
14.
CapuanoEFoglianoV (2011) Acrylamide and 5-hydroxymethylfurfural (HMF): A review on metabolism, toxicity, occurrence in food and mitigation strategies. LWT – Food Science and Technology44(4): 793–810.
15.
CárcelJAGarcía-PérezJVBeneditoJMuletA (2012) Food process innovation through new technologies: Use of ultrasound. Journal of Food Engineering110(2): 200–207.
16.
ChaikhamPKemsawasdVApichartsrangkoonA (2016) Effects of conventional and ultrasound treatments on physicochemical properties and antioxidant capacity of floral honeys from Northern Thailand. Food Bioscience15: 19–26.
17.
Chaikham P and Prangthip P. (2015). Alteration of antioxidative properties of longan flower-Honey after high pressure, ultra-sonic and thermal processing. Food Bioscience 10: 1–7.
18.
ChematFZill-e-HumaKhanMK (2011) Applications of ultrasound in food technology: Processing, preservation and extraction. Ultrasonics Sonochemistry18(4): 813–835.
19.
ChinNLTanMCChe PaNFYusofYA (2013) A Method and Apparatus for High Intensity Ultrasonic Treatment of Baking Materials, United States: Universiti Putra Malaysia.
20.
ChuttongBChanbangYSringarmKBurgettM (2016) Physicochemical profiles of stingless bee (Apidae: Meliponini) honey from South East Asia (Thailand). Food Chemistry192: 149–155.
21.
CorralesMToepflSButzPKnorrDTauscherB (2008) Extraction of anthocyanins from grape by-products assisted by ultrasonics, high hydrostatic pressure or pulsed electric fields: A comparison. Innovative Food Science & Emerging Technologies9(1): 85–91.
22.
CruzRMSVieiraMCSilvaCLM (2007) Modelling kinetics of watercress (Nasturtium officinale) colour changes due to heat and thermosonication treatments. Innovative Food Science & Emerging Technologies8(2): 244–252.
23.
EscricheIVisquertMJuan-BorrásMFitoP (2009) Influence of simulated industrial thermal treatments on the volatile fractions of different varieties of honey. Food Chemistry112(2): 329–338.
24.
FallicoBArenaEZappalaM (2008) Degradation of 5-hydroxymethylfurfural in honey. Journal of Food Science73(9): C625–C631.
25.
Fontana AJ Jr, Schmidt SJ and Labuza TP. (2008). Water Activity in Foods: Fundamentals and Applications (Vol. 13). Chichester: John Wiley & Sons.
26.
FrankelSRobinsonGEBerenbaumMR (1998) Antioxidant capacity and correlated characteristics of 14 unifloral honeys. Journal of Apicultural Research37(1): 27–31.
27.
GanesanBMartiniSSolorioJWalshMK (2015) Determining the effects of high intensity ultrasound on the reduction of microbes in milk and orange juice using response surface methodology. International Journal of Food Science2015: 350719.
GranatoDRibeiroJCBCastroIAMassonML (2010) Sensory evaluation and physicochemical optimisation of soy-based desserts using response surface methodology. Food Chemistry121(3): 899–906.
30.
GuanY-GZhangB-SYuS-JWangX-RXuX-BWangJet al. (2011) Effects of ultrasound on a glycin–glucose model system – A means of promoting maillard reaction. Food and Bioprocess Technology4(8): 1391–1398.
31.
GuerriniABruniRMaiettiSPoliFRossiDPaganettoGet al. (2009) Ecuadorian stingless bee (Meliponinae) honey: A chemical and functional profile of an ancient health product. Food Chemistry114(4): 1413–1420.
32.
HoseneyRC (1984) Chemical changes in carbohydrates produced by thermal processing. Journal of Chemical Education61(4): 308.
33.
Kek SP, Chin NL, Tan SW, Yusof YA and Chua LS. (2016). Classification of honey from its bee origin via chemical profiles and mineral content. Food Analytical Methods 10(1): 19–30.
34.
KekSPChinNLYusofYATanSWChuaLS (2014) Total phenolic contents and colour intensity of Malaysian honeys from the Apis spp. and Trigona spp. bees. Agriculture and Agricultural Science Procedia2: 150–155.
35.
KhalilMISulaimanSAGanSH (2010) High 5-hydroxymethylfurfural concentrations are found in Malaysian honey samples stored for more than one year. Food and Chemical Toxicology48(8–9): 2388–2392.
36.
LazaridouABiliaderisCGBacandritsosNSabatiniAG (2004) Composition, thermal and rheological behaviour of selected Greek honeys. Journal of Food Engineering64(1): 9–21.
37.
MarvinGEPetersonWHFredEBWilsonHF (1931) Some of the characteristics of yeasts found in fermenting honey. Journal of Agricultural Research43(2): 121–131.
38.
MasonTJ (1991) Practical Sonochemistry User’s Guide to Application in Chemistry and Chemical Engineering, UK: Ellis Horwood.
39.
MilaniEARamseyJGSilvaFVM (2016) High pressure processing and thermosonication of beer: Comparing the energy requirements and Saccharomyces cerevisiae ascospores inactivation with thermal processing and modeling. Journal of Food Engineering181: 35–41.
40.
MolyneuxP (2004) The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin Journal of Science and Technology26(2): 211–219.
41.
MoniruzzamanMKhalilMISulaimanSAGanSH (2013) Physicochemical and antioxidant properties of Malaysian honeys produced by Apis cerana, Apis dorsata and Apis mellifera. BMC Complementary and Alternative Medicine13(1): 1–12.
42.
MusielakGMierzwaDKroehnkeJ (2016) Food drying enhancement by ultrasound – A review. Trends in Food Science & Technology56: 126–141.
43.
NagaiTSakaiMInoueRInoueHSuzukiN (2001) Antioxidative activities of some commercially honeys, royal jelly, and propolis. Food Chemistry75(2): 237–240.
44.
NicoliMCAneseMParpinelMTFranceschiSLericiCR (1997) Loss and/or formation of antioxidants during food processing and storage. Cancer Letters114(1–2): 71–74.
45.
NociFWalkling-RibeiroMCroninDAMorganDJLyngJG (2009) Effect of thermosonication, pulsed electric field and their combination on inactivation of Listeria innocua in milk. International Dairy Journal19(1): 30–35.
46.
ÖrsiF (1973) Kinetic studies on the thermal decomposition of glucose and fructose. Journal of Thermal Analysis and Calorimetry5(2–3): 329–335.
47.
RaviyanPZhangZFengH (2005) Ultrasonication for tomato pectinmethylesterase inactivation: Effect of cavitation intensity and temperature on inactivation. Journal of Food Engineering70(2): 189–196.
48.
RawsonATiwariBKPatrasABruntonNBrennanCCullenPJet al. (2011) Effect of thermosonication on bioactive compounds in watermelon juice. Food Research International44(5): 1168–1173.
49.
Rodríguez-MalaverA (2013) Antioxidant activity of pot-honey. In: VitPPedroSRMRoubikD (eds) Pot-Honey, New York: Springer, pp. 475–480.
50.
SalaFJBurgosJCondónSLopezPRasoJ (1995) Effect of heat and ultrasound on microorganisms and enzymes. In: GouldGW (ed.) New Methods of Food Preservation, Boston, MA: Springer US, pp. 176–204.
51.
SestaGLuscoL (2008) Refractometric determination of water content in royal jelly. Apidologie39(2): 225–232.
52.
SilvaIAASouzaALCordeiroAMTMSoledadeLEBQueirozNSouzaAG (2013) Thermal degradation of honeys and evaluation of physicochemical properties. Journal of Thermal Analysis and Calorimetry114(1): 353–358.
53.
SingletonVLOrthoferRLamuela-RaventosRM (1999) Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology299C: 152–178.
54.
SuslickKS (1989) The chemical effects of ultrasound. Scientific American260(2): 80–86.
55.
TiwariBKMuthukumarappanKO’DonnellCPCullenPJ (2008) Colour degradation and quality parameters of sonicated orange juice using response surface methodology. LWT – Food Science and Technology41(10): 1876–1883.
56.
TiwariBKO’ DonnellCPMuthukumarappanKCullenPJ (2008) Effect of low temperature sonication on orange juice quality parameters using response surface methodology. Food and Bioprocess Technology2(1): 109–114.
57.
TiwariBKPatrasABruntonNCullenPJO’DonnellCP (2010) Effect of ultrasound processing on anthocyanins and color of red grape juice. Ultrasonics Sonochemistry17(3): 598–604.
58.
TurkmenNSariFPoyrazogluESVeliogluYS (2006) Effects of prolonged heating on antioxidant activity and colour of honey. Food Chemistry95(4): 653–657.
59.
van BoekelMAJS (2001) Kinetic aspects of the Maillard reaction: a critical review. Food/Nahrung45(3): 150–159.
60.
VitPBogdanovSKilchenmannV (1994) Composition of Venezuelan honeys from stingless bees (Apidae: Meliponinae) and Apis mellifera L. Apidologie25(3): 278–288.
61.
VitPMedinaMEunice EnríquezM (2004) Quality standards for medicinal uses of Meliponinae honey in Guatemala, Mexico and Venezuela. Bee World85(1): 2–5.
62.
WhiteJW (1979) Spectrophotometric method for hydroxymethylfurfural in honey. Journal – Association of Official Analytical Chemists62(3): 509–514.
63.
WhiteJWKushnirISubersMH (1964) Effect of storage and processing temperatures on honey quality. Food Technology18(4): 153–156.
64.
White JW Jr. (1978). Honey. In: Chichester CO (ed.) Advances in Food Research (Vol. 24). New York: Academic Press, pp.287–374.
65.
WuJGamageTVVilkhuKSSimonsLKMawsonR (2008) Effect of thermosonication on quality improvement of tomato juice. Innovative Food Science & Emerging Technologies9(2): 186–195.
66.
ZhaoLZhaoGChenFWangZWuJHuX (2006) Different effects of microwave and ultrasound on the stability of (all-E)-astaxanthin. Journal of Agricultural and Food Chemistry54(21): 8346–8351.
